Hi Patricio!
Generally, this approach: https://love2d.org/wiki/Tutorial:Isometric_Graphics
You could use Tiled editor to lay out your maps: https://www.youtube.com/watch?v=IL09GkElaoc
An isometric map loader has already been written, here: https://devforum.play.date/t/loading-maps-from-the-tiled-editor/4862/11
This gives you a good start!
On Playdate, you would want to leverage the Sprite system to avoid unnecessary redraws.
If your map is larger than the screen then you would need to do some optimisations so that only the visible tiles are drawn and processed for other things like animation and collision.
Testing this on hardware would be recommended, as there was a previous isometric game here https://devforum.play.date/t/nano-farm-farming-game-for-playdate/5668 that had performance problems (mostly from trying to do too much)